Journal: Endocrinology

Article Title: The temporal pattern of cfos activation in hypothalamic, cortical, and brainstem nuclei in response to fasting and refeeding in male mice.

doi: 10.1210/en.2013-1831

Figure Lengend Snippet: Figure 3. Refeeding increases cfos expression in the ARC and VMH. A, Fasting increased ARC cfos activation compared with that in nonfasted controls (0) and refeeding further increased cfos activation in the ARC at 1 and 2 hours after refeeding compared with that in 36-hour fasted mice. VMH cfos activation was increased at 0.5, 1, and 2 hours after refeeding compared with that in nonfasted controls. C, Representative images of the ARC and VMH from 36 hours fasted and 360.5, 361, and 362 hours refeeding. In the ARC panels, note the discrete anatomical shift in the cfos activation pattern from fasting to refeeding. D, Representative images from the rostral, middle, and caudal ARC show the anatomical localization of NPY (green) and POMC (red) neurons. NPY neurons are predominantly observed in the ventromedial portion of the ARC immediately adjacent to the third ventricle, whereas POMC neurons are predominantly lateral and dorsal to NPY neurons. All data are means SEM. a, Significant with respect to nonfasted controls (0); b, significant with respect to 36 hours fasted mice. We used one-way ANOVA followed by the Tukey post hoc test to determine statistical significance with GraphPad Prism. Scale bars are 100 m.

Article Snippet: Sections were incubated with cfos rabbit polyclonal IgG (sc-52; Santa Cruz Biotechnology, Inc) at 1:5000 in diluent of 1% normal horse serum in 0.3% Triton in 0.1 M PB.

Techniques: Expressing, Activation Assay

Journal: Endocrinology

Article Title: The temporal pattern of cfos activation in hypothalamic, cortical, and brainstem nuclei in response to fasting and refeeding in male mice.

doi: 10.1210/en.2013-1831

Figure Lengend Snippet: Figure 4. Activation of NPY and POMC neurons during fasting and refeeding. A, After sampling throughout the entire ARC, we observed that after 36 hours of fasting 25% of NPY neurons showed cfos activation, and this returned to fed levels within 1 hour of refeeding (n 6 per group). B, We also observed a significant increase in the number of POMC neurons expressing cfos at 1 and 2 hours after refeeding relative to that in fasted mice (n 6 per group). C, Representative images showing ARC POMC neurons (brown cytoplasm; yellow arrowheads), cfos-positive cells (black nuclei; white arrowheads), and POMC/cfos-positive neurons (brown/black; black arrows). D and E, Representative images of NPY GFP neurons in the ARC (green), cfos (red), and NPY GFP neurons expressing cfos (merge) after 36 hours of fasting and 1 hour of refeeding. All data are means SEM. a, Significant with respect to fed controls (A) or fasted mice (B). We used one-way ANOVA followed by the Tukey post hoc test to determine statistical significance with GraphPad Prism. Scale bars are 100 m.

Article Snippet: Sections were incubated with cfos rabbit polyclonal IgG (sc-52; Santa Cruz Biotechnology, Inc) at 1:5000 in diluent of 1% normal horse serum in 0.3% Triton in 0.1 M PB.

Techniques: Activation Assay, Sampling, Expressing

Journal: Endocrinology

Article Title: The temporal pattern of cfos activation in hypothalamic, cortical, and brainstem nuclei in response to fasting and refeeding in male mice.

doi: 10.1210/en.2013-1831

Figure Lengend Snippet: Figure 5. Refeeding increases cfos expression in the NAc and cingulate cortex. A, Acute refeeding within 2 hours but not after 6 hours increased cfos activation in the NAc compared with that in nonfasted controls. B, Acute feeding stimulated cfos expression in the cingulate cortex compared with that in nonfasted controls. C, No difference in cfos expression in the insular cortex was observed. D, Representative images of cfos expression in the NAc and cingulate cortex in nonfasted and refed mice for 0.5, 1, or 2 hours. All data are means sem. a, Significant with respect to nonfasted controls (0). We used one-way ANOVA followed by the Tukey post hoc test to determine statistical significance with GraphPad Prism. Scale bars are 100 m.

Article Snippet: Sections were incubated with cfos rabbit polyclonal IgG (sc-52; Santa Cruz Biotechnology, Inc) at 1:5000 in diluent of 1% normal horse serum in 0.3% Triton in 0.1 M PB.

Techniques: Expressing, Activation Assay

Journal: Endocrinology

Article Title: The temporal pattern of cfos activation in hypothalamic, cortical, and brainstem nuclei in response to fasting and refeeding in male mice.

doi: 10.1210/en.2013-1831

Figure Lengend Snippet: Figure 6. Refeeding increases cfos expression in the PBN. A, Acute refeeding activated cfos expression in the MPBN compared with that in nonfasted controls. B, Acute refeeding activated cfos expression in the LPBN compared with that in nonfasted controls. C, Refeeding caused greater cfos expression in the LPBN than in the MPBN. D, Representative images of cfos expression in the MPBN and LPBN in nonfasted and refed mice for 0.5, 1, or 2 hours. All data are means sem. a, Significant with respect to nonfasted controls (0). We used one-way ANOVA followed by the Tukey post hoc test to determine statistical significance with GraphPad Prism. Scale bars are 100 m. scp, superior cerebellar peduncle.

Article Snippet: Sections were incubated with cfos rabbit polyclonal IgG (sc-52; Santa Cruz Biotechnology, Inc) at 1:5000 in diluent of 1% normal horse serum in 0.3% Triton in 0.1 M PB.

Techniques: Expressing

Journal: Endocrinology

Article Title: The temporal pattern of cfos activation in hypothalamic, cortical, and brainstem nuclei in response to fasting and refeeding in male mice.

doi: 10.1210/en.2013-1831

Figure Lengend Snippet: Figure 7. Refeeding increases cfos expression in the NTS, AP, and DR. Acute refeeding activated cfos expression in the NTS compared with that in nonfasted controls. B, Acute refeeding activated cfos expression in the AP compared with that in nonfasted controls. C, Acute refeeding activated cfos expression the DR compared with that in nonfasted controls. D, Representative images of cfos expression in the NTS and AP and DR in nonfasted and refed mice for 0.5, 1, or 2 hours. All data are means SEM. a, Significant with respect to nonfasted controls (0). We used one-way ANOVA followed by the Tukey post hoc test to determine statistical significance with GraphPad Prism. Scale bars are 100 m.

Article Snippet: Sections were incubated with cfos rabbit polyclonal IgG (sc-52; Santa Cruz Biotechnology, Inc) at 1:5000 in diluent of 1% normal horse serum in 0.3% Triton in 0.1 M PB.

Techniques: Expressing

Journal: Endocrinology

Article Title: The temporal pattern of cfos activation in hypothalamic, cortical, and brainstem nuclei in response to fasting and refeeding in male mice.

doi: 10.1210/en.2013-1831

Figure Lengend Snippet: Figure 8. Fasting and feeding increase cfos expression in the amygdala. A, cfos expression in the central amygdala after fasting and refeeding. B, cfos expression in the medial amygdala after fasting and refeeding. C, cfos expression in the basolateral amygdala after fasting and refeeding. D, Representative images of cfos expression in the central, medial, and basolateral amygdala in nonfasted and refed mice for 0.5, 1, or 2 hours. All data are means SEM. a, Significant with respect to nonfasted controls (0). We used one-way ANOVA followed by the Tukey post hoc test to determine statistical significance with GraphPad Prism. Scale bars are 100 m.

Article Snippet: Sections were incubated with cfos rabbit polyclonal IgG (sc-52; Santa Cruz Biotechnology, Inc) at 1:5000 in diluent of 1% normal horse serum in 0.3% Triton in 0.1 M PB.

Techniques: Expressing